嗜盐古菌嗜盐栖热菌的高效转染
Efficient transfection of the archaebacterium Halobacterium halobium.
作者信息
Cline S W, Doolittle W F
出版信息
J Bacteriol. 1987 Mar;169(3):1341-4. doi: 10.1128/jb.169.3.1341-1344.1987.
Abstract
We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA. Between 0.5 and 1% of spheroplasts were transfected per microgram of luminal diameter H DNA. Under our conditions, survival and regeneration of H. halobium spheroplasts were also quite efficient, suggesting that this method will be useful for introducing other DNAs into these bacteria.
摘要
我们为极端嗜盐古细菌嗜盐栖热菌开发了一种高效的聚乙二醇介导的原生质球转染方法。59千碱基对的线性噬菌体φH DNA分子通常每微克DNA能产生5×10⁶至2×10⁷个转染子。每微克腔直径H DNA能转染0.5%至1%的原生质球。在我们的条件下，嗜盐栖热菌原生质球的存活和再生效率也相当高，这表明该方法将有助于将其他DNA导入这些细菌。
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本文引用的文献
1
Halobacterium halobium phage øH.嗜盐菌噬菌体 øH.
EMBO J. 1982;1(1):87-92. doi: 10.1002/j.1460-2075.1982.tb01129.x.
2
A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.一种用于聚乙二醇诱导细菌和酵母遗传转化的通用方法。
Gene. 1983 Nov;25(2-3):333-41. doi: 10.1016/0378-1119(83)90238-x.
3
Fractionation of DNA fragments by polyethylene glycol induced precipitation.通过聚乙二醇诱导沉淀对DNA片段进行分级分离。
Methods Enzymol. 1980;65(1):347-53. doi: 10.1016/s0076-6879(80)65044-7.
4
Lipid interactions in membranes of extremely halophilic bacteria. II. Modification of the bilayer structure by squalene.极端嗜盐细菌膜中的脂质相互作用。II. 角鲨烯对双层结构的修饰。
Biochemistry. 1974 Nov 19;13(24):4914-20. doi: 10.1021/bi00721a006.
5
Genetic transfer in Halobacterium volcanii.嗜盐嗜碱菌中的基因转移。
J Bacteriol. 1985 Apr;162(1):461-2. doi: 10.1128/jb.162.1.461-462.1985.
6
Structural (shape-maintaining) role of the cell surface glycoprotein of Halobacterium salinarium.盐生盐杆菌细胞表面糖蛋白的结构（维持形状）作用。
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2687-91. doi: 10.1073/pnas.73.8.2687.
7
Studies on the biosynthesis of bacterio-opsin. Demonstration of the existence of protein species structurally related to bacterio-opsin.细菌视紫红质生物合成的研究。与细菌视紫红质结构相关的蛋白质种类存在的证明。
Eur J Biochem. 1978 Aug 15;89(1):229-35. doi: 10.1111/j.1432-1033.1978.tb20917.x.
8
Phylogenetic structure of the prokaryotic domain: the primary kingdoms.原核生物域的系统发育结构：主要类群
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088-90. doi: 10.1073/pnas.74.11.5088.
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