Departments of Crop and Soil Sciences and Entomology, 202 Pesticide Research Center, Michigan State University, 48824-1311, East Lansing, MI, USA.
Plant Cell Rep. 1991 Nov;10(9):453-6. doi: 10.1007/BF00233813.
We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were evaluated. Callus produced on the Murashige and Skoog (MS) (1962) medium containing 2,4-D had low embryogenic potency. In the presence of 30 μM dicamba, addition of 2.25 to 9 μM BA significantly enhanced embryogenic callus formation over dicamba alone. Optimum frequency of somatic embryogenesis was achieved on MS basal medium containing 30 μM dicamba and 2.25 μM BA. Over 80% of somatic embryos germinated and formed plantlets on half-strength MS basal medium. These plantlets grew normally in the greenhouse.
我们通过成熟种子体细胞胚胎发生建立了一个高频的匍匐翦股颖（Agrostis palustris Huds）再生系统。评估了 2,4-二氯苯氧乙酸（2,4-D）、3.6-二氯邻氨基苯甲酸（dicamba）和 6-苄基腺嘌呤（BA）对愈伤组织形成和胚胎发生的影响。在含有 2,4-D 的 Murashige 和 Skoog (MS)（1962）培养基上产生的愈伤组织胚胎发生能力较低。在 30 μM dicamba 的存在下，添加 2.25 至 9 μM BA 显著增强了与 dicamba 单独处理相比的胚胎发生愈伤组织形成。在含有 30 μM dicamba 和 2.25 μM BA 的 MS 基本培养基上实现了最佳的体细胞胚胎发生频率。超过 80%的体细胞胚胎在半强度 MS 基本培养基上发芽并形成植株。这些植株在温室中正常生长。
