Daneels G, Moeremans M, De Raeymaeker M, De Mey J
J Immunol Methods. 1986 May 1;89(1):89-91. doi: 10.1016/0022-1759(86)90035-9.
A double staining method is described which combines immunodetection with sensitive staining of the complete electropherogram on the same membrane. The method is based on the use of Tween 20 as blocking agent, and uses immunogold/silver staining of specific antigens and gold staining of the overall protein pattern with AuroDye. This double staining makes possible the exact location of an immunodetected band within a complex protein pattern.
本文描述了一种双重染色方法，该方法将免疫检测与同一膜上完整电泳图谱的灵敏染色相结合。该方法基于使用吐温20作为封闭剂，并采用对特定抗原进行免疫金/银染色以及用AuroDye对总蛋白质图谱进行金染色。这种双重染色能够在复杂的蛋白质图谱中精确确定免疫检测条带的位置。
