Banaeian-Borujeni Shayeste, Mobini Gholam R, Pourgheysari Batoul, Validi Majid
Midwifery Department, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Adv Biomed Res. 2013 Jul 30;2:57. doi: 10.4103/2277-9175.115800. eCollection 2013.
One of the most common causes of vaginitis is candidiasis. The aim of this study is to compare the effect of honey and miconazole against Candida albicans, in vitro.
The different W/V concentrations of honey were prepared at 20, 40, 60, 80, and 95% and different dilutions of miconazole were prepared in 0.05, 5, and 50 μg/ml. A microdilution of 100/000 cells per ml of a two-day old culture of Candida albicans was prepared in normal saline, after culturing the strain of PTCC 5027 in RPMI 1640 medium. Ten microliters of this dilution was added to 1 ml of the RPMI 1640 medium containing different concentrations of honey and to 1 ml of the RPMI 1640 medium containing different dilutions of miconazole. The cultures were incubated at 35°C for 12, 24, and 48 hours.
The growth rate of Candida albicans was determined in the cultures. The results indicated that the honey prevented the growth of C. albicans greatly only at an 80% concentration, whereas, miconazole inhibited it completely.
As Candida albicans is a normal vaginal flora, the inhibitory effect of honey without the fungicide effect is a very good trend in the treatment of vaginal candidiasis.
阴道炎最常见的病因之一是念珠菌病。本研究的目的是在体外比较蜂蜜和咪康唑对白色念珠菌的作用。
制备20%、40%、60%、80%和95%不同重量/体积浓度的蜂蜜，并制备0.05μg/ml、5μg/ml和50μg/ml不同稀释度的咪康唑。将PTCC 5027菌株在RPMI 1640培养基中培养后，在生理盐水中制备每毫升含100/000个细胞的两天龄白色念珠菌培养物的微量稀释液。将10微升这种稀释液加入到1毫升含有不同浓度蜂蜜的RPMI 1640培养基中，以及1毫升含有不同稀释度咪康唑的RPMI 1640培养基中。培养物在35℃下孵育12小时、24小时和48小时。
测定培养物中白色念珠菌的生长速率。结果表明，蜂蜜仅在80%浓度时能极大地抑制白色念珠菌的生长，而咪康唑能完全抑制其生长。
由于白色念珠菌是正常的阴道菌群，蜂蜜的抑制作用而无杀菌作用在治疗阴道念珠菌病方面是一个非常好的趋势。
