Mohanty Indrani, Panda Pritilata, Sahu Susmita, Dash Mutikesh, Narasimham Moningi Venkat, Padhi Sanghamitra, Parida Banojini
Department of Microbiology, ART Centre, M.K.C.G. Medical College, Berhampur, Odisha, India.
Adv Biomed Res. 2013 Jul 30;2:61. doi: 10.4103/2277-9175.115810. eCollection 2013.
To determine the prevalence of Isospora belli and its correlation with CD4+ cell counts in HIV-positive patients with diarrhea in this region.
Stool samples from 250 HIV-positive patients, including 200 with diarrhea and 50 without diarrhea included in the study were examined for the presence of enteric parasites under microscopy. Prevalence of the enteric parasites with special reference to I. belli in HIV-positive patients with and without diarrhea were calculated and correlated with their CD4+ cell counts.
Enteric parasites were detected in 39% of the HIV patients with diarrhea compared to 30% without diarrhea. I. belli was detected in 22% of the patients with diarrhea and in 4% without diarrhea (P = 0.0019). I. belli was the most common parasite, followed by Entamoeba histolytica/dispar (8%) and Cryptosporidium parvum (5%) in HIV-positive patients with diarrhea. In HIV-positive patients without diarrhea, the most common parasite detected was E. histolytica/dispar (12%) followed by C. parvum (6%) and I. belli (4%). The mean CD4 cell count of HIV-positive patients with diarrhea suffering from isosporiasis was 138.35 ± 70.71. In patients with CD4 cell counts <200/μl, I. belli was seen in 36/123 stool samples and 2/27 stool samples which was statistically significant (P = 0.0157).
I. belli was the predominant parasite with a prevalence of 22% among HIV-positive patients with diarrhea, majority having CD4 cell count <200/μl. This study highlights the importance of routine screening for coccidian parasites in HIV-positive patients with and without diarrhea especially in those with low CD4 cell counts.
确定该地区腹泻的HIV阳性患者中贝氏等孢球虫的感染率及其与CD4+细胞计数的相关性。
对250例HIV阳性患者的粪便样本进行检查，其中包括200例腹泻患者和50例无腹泻患者，通过显微镜检查肠道寄生虫的存在情况。计算有腹泻和无腹泻的HIV阳性患者中肠道寄生虫的感染率，特别关注贝氏等孢球虫，并将其与CD4+细胞计数进行相关性分析。
腹泻的HIV患者中39%检测到肠道寄生虫，无腹泻的患者中这一比例为30%。腹泻患者中22%检测到贝氏等孢球虫，无腹泻患者中为4%（P = 0.0019）。在腹泻的HIV阳性患者中，贝氏等孢球虫是最常见的寄生虫，其次是溶组织内阿米巴/迪氏内阿米巴（8%）和微小隐孢子虫（5%）。在无腹泻的HIV阳性患者中，检测到的最常见寄生虫是溶组织内阿米巴/迪氏内阿米巴（12%），其次是微小隐孢子虫（6%）和贝氏等孢球虫（4%）。患等孢球虫病的腹泻HIV阳性患者的平均CD4细胞计数为138.35 ± 70.71。在CD4细胞计数<200/μl的患者中，123份粪便样本中有36份检测到贝氏等孢球虫，27份粪便样本中有2份检测到，差异具有统计学意义（P = 0.0157）。
贝氏等孢球虫是腹泻的HIV阳性患者中主要的寄生虫，感染率为22%，大多数患者的CD4细胞计数<200/μl。本研究强调了对有腹泻和无腹泻的HIV阳性患者，尤其是CD4细胞计数低的患者进行球虫寄生虫常规筛查的重要性。
