Ryan E D, Bilous G
Clin Biochem. 1983 Aug;16(4):249-53. doi: 10.1016/s0009-9120(83)90126-1.
We have modified a manual assay method for the determination of serum 5'-nucleotidase so that the reaction product, phosphate, is assayed colorimetrically using a continuous flow system. The contribution of non-specific phosphatase enzymes is assessed in the presence of nickel ions which specifically inhibit 5'-nucleotidase. The detergent sodium lauryl sulfate is used to eliminate the need for deproteinization in the phosphate assay. We have measured 5'-nucleotidase activity in the sera of 123 breast cancer patients and correlated our data with the patients' clinical status. Receiver operating characteristic curve analysis showed the most advantageous cut-off point for indication of secondary spread to be 10 U/L. Using this cut-off point, 14% of patients who were clinically free of disease and 35% of patients with disease clinically present had elevated 5'-nucleotidase activity.
我们改进了一种用于测定血清5'-核苷酸酶的手工检测方法，以便使用连续流动系统通过比色法检测反应产物磷酸盐。在镍离子存在的情况下评估非特异性磷酸酶的作用，镍离子可特异性抑制5'-核苷酸酶。使用十二烷基硫酸钠洗涤剂消除了磷酸盐检测中对蛋白质去除的需求。我们测定了123例乳腺癌患者血清中的5'-核苷酸酶活性，并将我们的数据与患者的临床状况相关联。受试者工作特征曲线分析表明，提示继发性扩散的最有利截断点为10 U/L。使用该截断点，临床无疾病的患者中有14%以及临床有疾病的患者中有35%的5'-核苷酸酶活性升高。
