从圭亚那柱花草、大约翰逊草和多花木兰幼苗子叶分离的原生质体再生植株。

Plant regeneration from protoplasts isolated from seedling cotyledons of Stylosanthes guianensis, S. macrocephala and S. scabra.

机构信息

Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG 2RD, Nottingham, UK.

出版信息

Plant Cell Rep. 1990 Sep;9(5):289-92. doi: 10.1007/BF00232304.

Abstract

Plant regeneration from protoplasts is a prerequisite to the production of modified plants using somatic hybridization and transformation. Whole plant regeneration was achieved from protoplasts isolated from seedling cotyledons of Stylosanthes guianensis, S. macrocephala and S. scabra, three economically important species of this tropical forage legume genus. The effects of both protoplast density and protoplast culture method on cell division and plating efficiency are presented.

摘要

原生质体再生是利用体细胞杂交和转化生产改良植物的前提。从热带饲料豆科植物圭亚那柱花草、大翼豆和四棱野豌豆的幼苗子叶分离的原生质体，实现了这三个重要经济种的植株再生。本文介绍了原生质体密度和原生质体培养方法对细胞分裂和平板效率的影响。