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从圭亚那柱花草、大约翰逊草和多花木兰幼苗子叶分离的原生质体再生植株。

Plant regeneration from protoplasts isolated from seedling cotyledons of Stylosanthes guianensis, S. macrocephala and S. scabra.

机构信息

Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG 2RD, Nottingham, UK.

出版信息

Plant Cell Rep. 1990 Sep;9(5):289-92. doi: 10.1007/BF00232304.

DOI:10.1007/BF00232304
PMID:24226829
Abstract

Plant regeneration from protoplasts is a prerequisite to the production of modified plants using somatic hybridization and transformation. Whole plant regeneration was achieved from protoplasts isolated from seedling cotyledons of Stylosanthes guianensis, S. macrocephala and S. scabra, three economically important species of this tropical forage legume genus. The effects of both protoplast density and protoplast culture method on cell division and plating efficiency are presented.

摘要

原生质体再生是利用体细胞杂交和转化生产改良植物的前提。从热带饲料豆科植物圭亚那柱花草、大翼豆和四棱野豌豆的幼苗子叶分离的原生质体，实现了这三个重要经济种的植株再生。本文介绍了原生质体密度和原生质体培养方法对细胞分裂和平板效率的影响。

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Plant regeneration from protoplasts isolated from seedling cotyledons of Stylosanthes guianensis, S. macrocephala and S. scabra.从圭亚那柱花草、大约翰逊草和多花木兰幼苗子叶分离的原生质体再生植株。
Plant Cell Rep. 1990 Sep;9(5):289-92. doi: 10.1007/BF00232304.
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Production and characterization of arboreous and fertile Solanum melongena + Solanum marginatum somatic hybrid plants.乔木状可育茄子+刺萼龙葵体细胞杂交植株的产生与特性分析
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Direct gene transfer in potato: a comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts.

本文引用的文献

1
Mesophyll protoplasts of fenugreek (Trigonella foenumgraecum): Isolation, culture and shoot regeneration.胡芦巴叶片原生质体：分离、培养和芽再生。
Plant Cell Rep. 1983 Jun;2(3):119-21. doi: 10.1007/BF00269333.
2
Prolific plant regeneration from protoplast-derived tissues of Lotus corniculatus L. (birdsfoot trefoil).从 Lotus corniculatus L.（鸟足三叶草）原生质体衍生组织中进行高效植物再生。
Plant Cell Rep. 1983 Apr;2(2):101-4. doi: 10.1007/BF00270177.
3
Somatic embryogenesis from mesophyll protoplasts of Trigonella corniculata (leguminosae).
马铃薯中的直接基因转移：叶片外植体粒子轰击与原生质体聚乙二醇介导转化的比较。
Plant Cell Rep. 2005 Dec;24(10):603-11. doi: 10.1007/s00299-005-0018-0. Epub 2005 Nov 16.
荠（豆科）叶肉原生质体体细胞胚胎发生。
Plant Cell Rep. 1982 Dec;1(6):278-80. doi: 10.1007/BF00272639.
4
Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. A tropical forage legume.在圭亚那猪殃殃（一种热带豆科饲料作物）中，分离的叶肉和细胞悬浮原生质体再生成植株。
Plant Cell Rep. 1986 Jun;5(3):174-7. doi: 10.1007/BF00269111.
5
Production of somatic hybrid tissues following chemical and electrical fusion of protoplasts from albino cell suspensions ofMedicago sativa andM. borealis.通过化学和电融合法将紫花苜蓿和北极苜蓿白化细胞悬浮原生质体融合，产生体细胞杂种组织。
Plant Cell Rep. 1989 May;8(1):29-32. doi: 10.1007/BF00735772.
6
The isolation, culture and regeneration of Petunia leaf protoplasts.矮牵牛叶片原生质体的分离、培养与再生
Dev Biol. 1973 Jul;33(1):130-7. doi: 10.1016/0012-1606(73)90169-3.