Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK.
Plant Cell Rep. 1982 Dec;1(6):278-80. doi: 10.1007/BF00272639.
Mesophyll protoplasts isolated enzymatically from Trigonella corniculata divided to form callus, with a plating efficiency of 49% in Kao (1977) medium. Protoplast-derived tissues formed somatic embryoids at high frequency on MS medium with 2.0 mg L(-7) NAA and 0.5 mg L(-7) BAP. Embryoids developed into plants on MS medium lacking hormones.
从蒺藜苜蓿中分离出的叶肉原生质体在 Kao（1977）培养基中通过酶解进行分裂，形成愈伤组织，其接种效率为 49%。在 MS 培养基中添加 2.0mg/L NAA 和 0.5mg/L BAP，原生质体衍生的组织高频形成体细胞胚。胚状体在不含激素的 MS 培养基上发育成植株。
