从 Lotus corniculatus L.（鸟足三叶草）原生质体衍生组织中进行高效植物再生。
Prolific plant regeneration from protoplast-derived tissues of Lotus corniculatus L. (birdsfoot trefoil).
机构信息
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
出版信息
Plant Cell Rep. 1983 Apr;2(2):101-4. doi: 10.1007/BF00270177.
PMID:24257960
Abstract
Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manipulation.
摘要
从莲（Nelumbo nucifera Gaertn.）幼苗的根、下胚轴和子叶中通过酶解法分离得到的原生质体可产生愈伤组织，进而经历大量的芽再生。从原生质体衍生组织中快速、轻松地回收植物，使这种饲料豆科植物成为遗传操作的有吸引力的实验系统。
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