Becker Marc, Lorenz Steffen, Strand Dennis, Vahl Christian-Friedrich, Gabriel Matthias
I. Medical Department, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
ScientificWorldJournal. 2013 Oct 21;2013:616535. doi: 10.1155/2013/616535. eCollection 2013.
In recent years, the synthetic polymer polyetheretherketone (PEEK) has increasingly been used in a number of orthopedic implementations, due to its excellent mechanical properties, bioinertness, and chemical resistance. For in vivo applications, the surface of PEEK, which does not naturally support cell adhesion, has to be modified to improve tissue integration. In the present work we demonstrate a novel wet-chemical modification of PEEK to modify the surface, enabling the covalent grafting of the cell-adhesive RGD-peptide. Modification of the polymer surface was achieved via Schiff base formation using an aliphatic diamine and subsequent crosslinker-mediated immobilization of the peptide. In cell culture experiments with primary osteoblasts it was shown that the RGD-modified PEEK not only significantly promoted cellular adhesion but also strongly enhanced the proliferation of osteoblasts on the modified polymer surface.
近年来，合成聚合物聚醚醚酮（PEEK）因其优异的机械性能、生物惰性和耐化学性，越来越多地用于多种骨科植入物中。对于体内应用，由于PEEK表面天然不支持细胞黏附，因此必须对其进行改性以改善组织整合。在本研究中，我们展示了一种新颖的PEEK湿化学改性方法来修饰其表面，从而实现细胞黏附性RGD肽的共价接枝。通过使用脂肪族二胺形成席夫碱并随后通过交联剂介导固定肽来实现聚合物表面的改性。在原代成骨细胞的细胞培养实验中表明，RGD改性的PEEK不仅显著促进了细胞黏附，而且还强烈增强了成骨细胞在改性聚合物表面的增殖。
