Briem D, Strametz S, Schröder K, Meenen N M, Lehmann W, Linhart W, Ohl A, Rueger J M
Department of Trauma-, Hand- and Reconstructive Surgery, School of Medicine, Hamburg University, Martinistr. 52, D-20246, Hamburg, Germany.
J Mater Sci Mater Med. 2005 Jul;16(7):671-7. doi: 10.1007/s10856-005-2539-z.
Polyetheretherketone (PEEK) is a synthetic polymer with suitable biomechanical and stable chemical properties, which make it attractive for use as an endoprothetic material and for ligamentous replacement. However, chemical surface inertness does not account for a good interfacial biocompatibility, and PEEK requires a surface modification prior to its application in vivo. In the course of this experimental study we analyzed the influence of plasma treatment of PEEK surfaces on the cell proliferation and differentiation of primary fibroblasts and osteoblasts. Further we examined the possibility of inducing microstructured cell growth on a surface with plasma-induced chemical micropatterning. We were able to demonstrate that the surface treatment of PEEK with a low-temperature plasma has significant effects on the proliferation of fibroblasts. Depending on the surface treatment, the proliferation rate can either be stimulated or suppressed. The behavior of the osteoblasts was examined by evaluating differentiation parameters. By detection of alkaline phosphatase, collagen I, and mineralized extracellular matrix as parameters for osteoblastic differentiation, the examined materials showed results comparable to commercially available polymer cell culture materials such as tissue culture polystyrene (TCPS). Further microstructured cell growth was produced successfully on micropatterned PEEK foils, which could be a future tool for bioartificial systems applying the methods of tissue engineering. These results show that chemically inert materials such as PEEK may be modified specifically through the methods of plasma technology in order to improve biocompatibility.
聚醚醚酮（PEEK）是一种具有合适生物力学性能和稳定化学性质的合成聚合物，这使其成为用作内置假体材料和韧带替代物的理想选择。然而，化学表面惰性并不意味着具有良好的界面生物相容性，PEEK在体内应用之前需要进行表面改性。在本实验研究过程中，我们分析了PEEK表面的等离子体处理对原代成纤维细胞和成骨细胞增殖及分化的影响。此外，我们研究了通过等离子体诱导化学微图案化在表面诱导微结构化细胞生长的可能性。我们能够证明，用低温等离子体对PEEK进行表面处理对成纤维细胞的增殖有显著影响。根据表面处理的不同，增殖率既可以被刺激也可以被抑制。通过评估分化参数来检测成骨细胞的行为。通过检测碱性磷酸酶、I型胶原蛋白和矿化细胞外基质作为成骨细胞分化的参数，所检测的材料显示出与市售聚合物细胞培养材料如组织培养聚苯乙烯（TCPS）相当的结果。此外，在微图案化的PEEK箔片上成功产生了微结构化细胞生长，这可能成为应用组织工程方法的生物人工系统的未来工具。这些结果表明，诸如PEEK之类的化学惰性材料可以通过等离子体技术方法进行特定改性，以提高生物相容性。
