Piper P J, Seale J P
Br J Pharmacol. 1979 Sep;67(1):67-72.
1 During incubation with calcium ionophore A23187, sensitized and unsensitized guinea-pig chopped lung released a slow-reacting substance (SRS) and histamine. 2 SRS possessed many characteristics of slow-reacting substance of anaphylaxis (SRS-A). It was inactivated by arylsulphatase, antagonized by FPL55712 (1 microgram/ml) and more stable in alkali than acid. Furthermore, it behaved like SRS-A by stimulating archidonic acid metabolism in guinea-pig isolated perfused lungs. 3 Maximal ionophore generation of SRS in sensitized lung was greater than maximal anaphylactic generation of SRS-A but the release of histamine was not significantly different. Simultaneous challenge with antigen and ionophore produced more SRS-like or SRS-A-like activity than either stimulus alone. 4 These results have shown a non-immunological release from guinea-pig lung of SRS which was similar (or possibly identical) to SRS-A. It is suggested that in addition to mast cells, other cell types may be involved.
1 在与钙离子载体A23187共同孵育期间，致敏和未致敏的豚鼠肺匀浆释放出一种慢反应物质（SRS）和组胺。2 SRS具有过敏反应慢反应物质（SRS-A）的许多特性。它可被芳基硫酸酯酶灭活，被FPL55712（1微克/毫升）拮抗，在碱性环境中比在酸性环境中更稳定。此外，它在刺激豚鼠离体灌注肺中的花生四烯酸代谢方面表现得与SRS-A相似。3 致敏肺中SRS的最大离子载体诱导生成量大于SRS-A的最大过敏反应生成量，但组胺的释放没有显著差异。抗原和离子载体同时刺激产生的SRS样或SRS-A样活性比单独使用任何一种刺激物都要高。4 这些结果表明，豚鼠肺可非免疫性释放出与SRS-A相似（或可能相同）的SRS。提示除肥大细胞外，其他细胞类型可能也参与其中。
