两个温度敏感型polA突变体：探究DNA聚合酶I在体内作用的一种方法。
Two temperature-sensitive polA mutants: an approach to the role in vivo of DNA polymerase I.
作者信息
Uyemura D G, Konrad E B, Lehman I R
出版信息
Basic Life Sci. 1975;5A:209-11. doi: 10.1007/978-1-4684-2895-7_27.
PMID:242313
Abstract
DNA polymerase I (Pol I) purified from a strain carrying polA12 demonstrates a defect primarily in the polymerase portion of the molecule. The mutant enzyme is altered in its structure and template specificity. Pol I isolated from strains carrying polAexl are primarily defective in 5' in equilibrium 3' exonuclease. The polAexl lesion renders strains conditonally lethal.
摘要
从携带polA12的菌株中纯化得到的DNA聚合酶I（Pol I）主要在分子的聚合酶部分表现出缺陷。突变酶的结构和模板特异性发生了改变。从携带polAexl的菌株中分离出的Pol I在5'至3'外切核酸酶方面存在主要缺陷。polAexl损伤使菌株具有条件致死性。
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