在具有温度敏感性脱氧核糖核酸聚合酶I的大肠杆菌K-12菌株的recA和recB衍生物中的脱氧核糖核酸合成
Deoxyribonucleic acid synthesis in recA and recB derivatives of an Escherichia coli K-12 strain with a temperature-sensitive deoxyribonucleic acid polymerase I.
作者信息
Monk M, Kinross J, Town C
出版信息
J Bacteriol. 1973 Jun;114(3):1014-7. doi: 10.1128/jb.114.3.1014-1017.1973.
Abstract
recA and recB derivatives of a strain of Escherichia coli with a temperature-sensitive deoxyribonucleic acid (DNA) polymerase I (polA12) are inviable at high temperature, but continue to incorporate (3)H-thymine into DNA for extended periods. The DNA made in pulse-chase experiments at high temperature in the polA12 parent and its double-mutant derivatives has been examined by alkaline sucrose gradient sedimentation analysis. The low-molecular-weight DNA fragments made during short pulses were joined at the same rate in each strain. Furthermore, the resulting high-molecular-weight DNA was of the same size in each case and was stable for at least 50 min. It is concluded that the inviability of the double mutants is due neither to a defect in converting low-molecular-weight DNA intermediates to high molecular weight nor to the presence of unrepaired random breaks in their DNA.
摘要
一株带有温度敏感型脱氧核糖核酸（DNA）聚合酶I（polA12）的大肠杆菌的recA和recB衍生物在高温下无法存活，但能在较长时间内持续将（3）H-胸腺嘧啶掺入DNA。通过碱性蔗糖梯度沉降分析，对polA12亲本及其双突变衍生物在高温下进行脉冲追踪实验所产生的DNA进行了检测。在每个菌株中，短脉冲期间产生的低分子量DNA片段以相同的速率连接。此外，在每种情况下，所产生的高分子量DNA大小相同，并且至少稳定50分钟。得出的结论是，双突变体的不可存活性既不是由于将低分子量DNA中间体转化为高分子量的缺陷，也不是由于其DNA中存在未修复的随机断裂。
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