Rees R C, Vallely P, Clegg A, Potter C W
Clin Exp Immunol. 1986 Mar;63(3):687-95.
The influence of human seminal plasma (SP) and whole semen (S) on the expression of natural cytotoxicity by human peripheral blood mononuclear cells (PBMNC) was examined. Marked suppression of natural cytotoxicity against K562 targets was observed when effectors were pre-treated for 1 h with SP or S diluted up to 1:400. Abrogation of cytolytic activity by SP was not the result of direct lymphotoxicity, although a reduction of approximately 50% in the number of target binding cells was observed. In addition, the cytotoxicity of interferon (alpha-IFN, beta-IFN, gamma-IFN) and interleukin 2 (IL-2) activated human PBMNC was suppressed by components present in human SP, although IL-2 activated human PBMC were relatively resistant to suppression compared with other effector (spontaneous or activated) populations. Following 1 h exposure to SP, PBMNC failed to recover more than 25% of their initial cytotoxic potential upon further in vitro incubation (18 h) in the absence of SP. However, both interferon and IL-2 caused an increase in the cytotoxicity of these populations, in some instances to the level obtained with control, IFN or IL-2 activated PBMNC. The biological significance of SP as an inhibitor of immune function under experimentally defined conditions is discussed in relation to its possible role in vivo.
研究了人类精浆（SP）和全精液（S）对人外周血单个核细胞（PBMNC）天然细胞毒性表达的影响。当效应细胞用稀释至1:400的SP或S预处理1小时后，观察到对K562靶标的天然细胞毒性受到明显抑制。尽管观察到靶标结合细胞数量减少了约50%，但SP消除细胞溶解活性并非直接淋巴毒性的结果。此外，人SP中的成分可抑制干扰素（α-干扰素、β-干扰素、γ-干扰素）和白细胞介素2（IL-2）激活的人PBMNC的细胞毒性，尽管与其他效应细胞（自发或激活）群体相比，IL-2激活的人PBMC对抑制作用相对具有抗性。在暴露于SP 1小时后，PBMNC在无SP的情况下进一步体外培养（18小时）时，未能恢复其初始细胞毒性潜能的25%以上。然而，干扰素和IL-2均导致这些群体的细胞毒性增加，在某些情况下达到与对照、干扰素或IL-2激活的PBMNC相同的水平。结合其在体内可能发挥的作用，讨论了在实验确定的条件下SP作为免疫功能抑制剂的生物学意义。
