CSIRO Division of Tropical Crops and Pastures, CSIRO-UQ Plant Pathology Unit, Department of Botany, University of Queensland, St. Lucia, 4067, Brisbane, Australia.
Plant Cell Rep. 1989 Jun;8(6):341-5. doi: 10.1007/BF00716669.
Agrobacterium rhizogenes carrying the binary Ti plasmid vector pGA492 was used to transform leaf and stem explants of the tropical pasture legumeStylosanthes humilis. Conditions which yielded kanamycin resistant roots at a frequency of up to 86% and subsequent plant regeneration at a frequency of 23% were defined. Transgenic plants were fertile and either grew normally or had stunted growth but otherwise showed only minor morphological abnormalities. Transgenic plants with normal phenotypes were obtained in the progeny of the primary regenerants. The presence of active neomycin phosphotransferase enzyme activity and binary vector DNA and TL-DNA was demonstrated in the regenerated plants. Evidence for the independent transfer of binary vector and TL-DNA was also obtained. This high frequency production of transgenic plants ofS. humilis is a major improvement over previous methods using disarmed strains ofA. tumefaciens as helper.
发根农杆菌携带二元 Ti 质粒载体 pGA492 被用于转化热带牧草豇豆的叶片和茎外植体。确定了能以高达 86%的频率产生卡那霉素抗性根，且随后以 23%的频率进行植物再生的条件。转基因植物可育，要么正常生长，要么生长迟缓，但除此之外，只有轻微的形态异常。在初代再生体的后代中获得了具有正常表型的转基因植物。在再生植物中证明了活性新霉素磷酸转移酶酶活性和二元载体 DNA 和 TL-DNA 的存在。还获得了二元载体和 TL-DNA 独立转移的证据。与以前使用无致病力的根癌农杆菌菌株作为辅助物的方法相比，豇豆的这种高效转基因植物的生产是一个重大改进。
