Spevak W, Hartig A, Meindl P, Ruis H
Mol Gen Genet. 1986 Apr;203(1):73-8. doi: 10.1007/BF00330386.
The 5'-flanking region of the Saccharomyces cerevisiae catalase T gene (CTT1) and the part of the gene coding for the N-terminus of catalase T were sequenced. 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension. To analyse control elements in the upstream region, a CTT1-lacZ gene fusion was constructed. Deletion analysis was carried out within a part of the 5'-flanking region showing homology to the upstream region of the yeast CYC1 gene. Like the CTT1 gene, this gene is controlled by heme, oxygen and glucose. The results obtained show that the CTT1 gene is positively controlled by heme. Tentative evidence has been obtained for the involvement of upstream sequences homologous to UAS1 and UAS2 of the CYC1 gene in heme control. Further, a negative site has been located between the upstream activator sites and the transcription start. Within this negative region a ten base-pair sequence was detected that shows high homology to a sequence located within a negative control region of the CYC1 gene and some homology to the negative control elements of the S. cerevisiae CAR1 and CAR2 genes.
对酿酒酵母过氧化氢酶T基因（CTT1）的5'侧翼区域以及编码过氧化氢酶T N端的部分基因进行了测序。通过S1核酸酶图谱分析和引物延伸确定了该区域转录本的5'末端。为了分析上游区域的调控元件，构建了CTT1 - lacZ基因融合体。在5'侧翼区域与酵母CYC1基因上游区域具有同源性的一部分内进行了缺失分析。与CTT1基因一样，该基因受血红素、氧气和葡萄糖的调控。所得结果表明CTT1基因受血红素的正向调控。已获得初步证据表明，与CYC1基因的UAS1和UAS2同源的上游序列参与了血红素调控。此外，在上游激活位点和转录起始点之间定位到了一个负调控位点。在这个负调控区域内检测到一个十碱基对序列，它与CYC1基因负调控区域内的一个序列具有高度同源性，并且与酿酒酵母CAR1和CAR2基因的负调控元件有一些同源性。
