酿酒酵母磷酸可阻遏酸性磷酸酶基因（PHO5）转录调控相关DNA序列的分子分析。
Molecular analysis of the DNA sequences involved in the transcriptional regulation of the phosphate-repressible acid phosphatase gene (PHO5) of Saccharomyces cerevisiae.
作者信息
Bergman L W, McClinton D C, Madden S L, Preis L H
出版信息
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6070-4. doi: 10.1073/pnas.83.16.6070.
Abstract
The expression of the PHO5 gene of Saccharomyces cerevisiae is transcriptionally regulated in response to the level of inorganic phosphate present in the growth medium. We have identified, by DNA deletion analysis, the sequences (upstream activator sequences) that mediate this response. The sequence 5' CTGCACAAATG 3' is present in two copies located within a 60-base-pair region. The presence of a single copy of the sequence is sufficient for the phosphate-mediated transcriptional response. In addition, a DNA fragment that contains two copies of this sequence will act to repress transcription of a CYC1-lacZ fusion when placed either upstream or downstream of the CYC1 activator sequence.
摘要
酿酒酵母PHO5基因的表达受生长培养基中无机磷酸盐水平的转录调控。我们通过DNA缺失分析确定了介导这种反应的序列（上游激活序列）。序列5' CTGCACAAATG 3' 以两个拷贝的形式存在于一个60碱基对区域内。该序列的单个拷贝的存在足以介导磷酸盐介导的转录反应。此外，一个包含该序列两个拷贝的DNA片段，当置于CYC1激活序列的上游或下游时，将抑制CYC1-lacZ融合基因的转录。
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