Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, Spain.
Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, Spain.
Comp Biochem Physiol A Mol Integr Physiol. 2014 Feb;168:40-9. doi: 10.1016/j.cbpa.2013.10.027. Epub 2013 Nov 13.
To elucidate the short-term time-course of liver metabolic response in rainbow trout to acute handling stress we subjected rainbow trout to 5min chasing and obtained samples 0 to 480min post-stress. Levels of cortisol, glucose and lactate were measured in plasma, whereas metabolite levels, enzyme activities, mRNA abundance of parameters related to energy metabolism, and glucocorticoid receptors were assessed in liver. Acute stress affected many parameters related to energy metabolism, with most of them turning back to normal levels after 480min. In general, the present results support the existence of two stages in the short-term time-course of metabolic response to handling stress. A first stage occurring few minutes post-stress (15-45min), was characterized by increased mobilization of liver glycogen resulting in increased production of endogenous glucose, reduced use of exogenous glucose and reduced lipogenic potential. A second stage, occurring 60-120min post-stress onwards was characterized by the recovery of liver glycogen levels, the increased capacity of liver for releasing glucose, and the recovery of lipogenic capacity whereas no changes were noted in gluconeogenic potential, which probably needs longer time periods to become enhanced.
为了阐明虹鳟鱼肝脏对急性处理应激的短期时间过程中的代谢反应，我们将虹鳟鱼追逐 5 分钟，并在应激后 0 至 480 分钟时获得样本。测量了血浆中的皮质醇、葡萄糖和乳酸水平，而肝脏中的代谢物水平、酶活性、与能量代谢相关的参数的 mRNA 丰度和糖皮质激素受体也进行了评估。急性应激影响了许多与能量代谢相关的参数，其中大多数在 480 分钟后恢复到正常水平。总的来说，目前的结果支持在处理应激的短期时间过程中存在两个代谢反应阶段。第一阶段发生在应激后几分钟内（15-45 分钟），其特征是肝脏糖原动员增加，导致内源性葡萄糖生成增加，外源性葡萄糖利用减少，脂肪生成潜力降低。第二阶段发生在应激后 60-120 分钟，其特征是肝脏糖原水平恢复，肝脏释放葡萄糖的能力增加，脂肪生成能力恢复，而糖异生潜力没有变化，这可能需要更长的时间才能增强。
