大鼠外分泌胰腺中的细胞特异性增强子。
Cell-specific enhancers in the rat exocrine pancreas.
作者信息
Boulet A M, Erwin C R, Rutter W J
出版信息
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3599-603. doi: 10.1073/pnas.83.11.3599.
Abstract
Pancreatic amylase, chymotrypsin B, and trypsin I genes are specifically expressed in the exocrine pancreas. The 5'-flanking regions of these genes direct preferential expression of a linked reporter function (chloramphenicol acetyltransferase) in the pancreatic exocrine cell line AR4-2J. The sequences upstream of the amylase and chymotrypsin genes that are required for this cell specific activity possess the characteristics of transcriptional enhancers. We have mapped the regions responsible for enhancer activity by deletion analysis. Modification of specific nucleotide sequences within these regions can alter or eliminate enhancer function. Comparison of the 5'-flanking regions of nine genes expressed in the exocrine pancreas identifies a family of short related sequences. These sequences are located within the enhancer regions that we have mapped and may play a role in the regulation of the expression of pancreatic exocrine-specific genes.
摘要
胰腺淀粉酶、胰凝乳蛋白酶B和胰蛋白酶I基因在外分泌胰腺中特异性表达。这些基因的5'侧翼区域可在胰腺外分泌细胞系AR4-2J中指导一个相连的报告功能（氯霉素乙酰转移酶）的优先表达。淀粉酶和胰凝乳蛋白酶基因上游对于这种细胞特异性活性所必需的序列具有转录增强子的特征。我们通过缺失分析确定了负责增强子活性的区域。这些区域内特定核苷酸序列的改变可改变或消除增强子功能。对在外分泌胰腺中表达的九个基因的5'侧翼区域进行比较，确定了一个短相关序列家族。这些序列位于我们所确定的增强子区域内，可能在胰腺外分泌特异性基因表达的调控中发挥作用。
相似文献
1
Cell-specific enhancers in the rat exocrine pancreas.大鼠外分泌胰腺中的细胞特异性增强子。
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3599-603. doi: 10.1073/pnas.83.11.3599.
2
Selective regulation of trypsin gene expression by calcium and by glucose starvation in a rat exocrine pancreas cell line.
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4292-6. doi: 10.1073/pnas.83.12.4292.
3
Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements.大鼠胰岛素基因的细胞特异性表达：两个不同的5'侧翼元件作用的证据。
Science. 1985 Nov 22;230(4728):912-6. doi: 10.1126/science.3904002.
4
Cell-specific enhancer activity in a far upstream region of the human alpha-fetoprotein gene.人类甲胎蛋白基因远上游区域中的细胞特异性增强子活性。
J Biol Chem. 1987 Apr 5;262(10):4812-8.
5
The chymotrypsin enhancer core. Specific factor binding and biological activity.
J Biol Chem. 1989 Dec 5;264(34):20744-51.
6
Upstream promoter element of the human metallothionein-IIA gene can act like an enhancer element.人类金属硫蛋白-IIA基因的上游启动子元件可发挥增强子元件的作用。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8572-6. doi: 10.1073/pnas.82.24.8572.
7
An endocrine-specific element is an integral component of an exocrine-specific pancreatic enhancer.一种内分泌特异性元件是外分泌特异性胰腺增强子的一个组成部分。
Genes Dev. 1993 May;7(5):774-86. doi: 10.1101/gad.7.5.774.
8
Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes.由胰岛素基因和胰凝乳蛋白酶基因的5'侧翼区域控制的细胞特异性表达。
Nature. 1983;306(5943):557-61. doi: 10.1038/306557a0.
9
A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene.在HLA - DRα基因的基因体内发现了一种组织特异性转录增强子。
J Exp Med. 1987 Sep 1;166(3):625-36. doi: 10.1084/jem.166.3.625.
10
A pancreatic exocrine cell factor and AP4 bind overlapping sites in the amylase 2A enhancer.
Biochemistry. 1991 Aug 20;30(33):8102-8. doi: 10.1021/bi00247a002.
引用本文的文献
1
Cloning of the gene and cDNA for hamster chymase 2, and expression of chymase 1, chymase 2 and angiotensin-converting enzyme in the terminal stage of cardiomyopathic hearts.仓鼠糜蛋白酶2基因和cDNA的克隆，以及糜蛋白酶1、糜蛋白酶2和血管紧张素转换酶在心肌病晚期心脏中的表达。
Biochem J. 1998 Jul 15;333 ( Pt 2)(Pt 2):417-24. doi: 10.1042/bj3330417.
2
Chicken vigilin gene: a distinctive pattern of hypersensitive sites is characteristic for its transcriptional activity.鸡维吉宁基因：其转录活性具有独特的超敏位点模式。
Biochem J. 1997 Sep 1;326 ( Pt 2)(Pt 2):601-7. doi: 10.1042/bj3260601.
3
4
A single element of the elastase I enhancer is sufficient to direct transcription selectively to the pancreas and gut.弹性蛋白酶I增强子的单个元件足以将转录选择性地导向胰腺和肠道。
Mol Cell Biol. 1994 Mar;14(3):2048-57. doi: 10.1128/mcb.14.3.2048-2057.1994.
5
Cooperation between elements of an organ-specific transcriptional enhancer in animals.动物器官特异性转录增强子元件之间的合作。
Mol Cell Biol. 1995 Aug;15(8):4385-94. doi: 10.1128/MCB.15.8.4385.
6
Identification of a transcriptional regulatory region of the rat pancreatitis-associated protein I (PAP I) gene that confers tissue specificity.大鼠胰腺炎相关蛋白I（PAP I）基因转录调控区域的鉴定，该区域赋予组织特异性。
Biochem J. 1995 Oct 15;311 ( Pt 2)(Pt 2):643-7. doi: 10.1042/bj3110643.
7
Crystallin genes: lens specificity of the murine alpha A-crystallin gene.晶状体蛋白基因：小鼠αA-晶状体蛋白基因的晶状体特异性
Environ Health Perspect. 1987 Nov;75:17-24. doi: 10.1289/ehp.877517.
8
Promoter and enhancer elements from the rat elastase I gene function independently of each other and of heterologous enhancers.大鼠弹性蛋白酶I基因的启动子和增强子元件相互独立发挥作用，且独立于异源增强子。
Mol Cell Biol. 1987 Oct;7(10):3466-72. doi: 10.1128/mcb.7.10.3466-3472.1987.
9
The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice.大鼠弹性蛋白酶I调控元件是一种增强子，可在转基因小鼠中指导正确的细胞特异性和表达的发育起始。
Mol Cell Biol. 1987 Aug;7(8):2956-67. doi: 10.1128/mcb.7.8.2956-2967.1987.
10
Sequence organisation and transcriptional regulation of the mouse elastase II and trypsin genes.小鼠弹性蛋白酶II和胰蛋白酶基因的序列组织与转录调控
Nucleic Acids Res. 1986 Nov 11;14(21):8307-30. doi: 10.1093/nar/14.21.8307.
本文引用的文献
1
Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.转染至淋巴细胞中的免疫球蛋白重链基因的表达与调控
EMBO J. 1983;2(8):1373-8. doi: 10.1002/j.1460-2075.1983.tb01594.x.
2
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.5S RNA基因中心的一个控制区域指导转录的特异性起始：I. 该区域的5'边界。
Cell. 1980 Jan;19(1):13-25. doi: 10.1016/0092-8674(80)90384-0.
3
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
4
A lymphocyte-specific enhancer in the mouse immunoglobulin kappa gene.小鼠免疫球蛋白κ基因中的淋巴细胞特异性增强子。
Nature. 1984;307(5946):80-2. doi: 10.1038/307080a0.
5
Immunoglobulin gene transcription is activated by downstream sequence elements.免疫球蛋白基因转录由下游序列元件激活。
Cell. 1983 Jul;33(3):741-8. doi: 10.1016/0092-8674(83)90016-8.
6
A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.一种组织特异性转录增强子元件位于重排的免疫球蛋白重链基因的主要内含子中。
Cell. 1983 Jul;33(3):717-28. doi: 10.1016/0092-8674(83)90014-4.
7
Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes.由胰岛素基因和胰凝乳蛋白酶基因的5'侧翼区域控制的细胞特异性表达。
Nature. 1983;306(5943):557-61. doi: 10.1038/306557a0.
8
Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations.猿猴病毒40早期和晚期区域启动子功能可通过插入到远处位置且方向相反的72碱基对重复序列得到增强。
Mol Cell Biol. 1983 Jun;3(6):991-9. doi: 10.1128/mcb.3.6.991-999.1983.
9
The SV40 72 bp repeat preferentially potentiates transcription starting from proximal natural or substitute promoter elements.SV40 72碱基对重复序列优先增强从近端天然或替代启动子元件起始的转录。
Cell. 1983 Feb;32(2):503-14. doi: 10.1016/0092-8674(83)90470-1.
10
The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.劳氏肉瘤病毒长末端重复序列通过DNA介导转染导入多种真核细胞时是一个强启动子。
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81. doi: 10.1073/pnas.79.22.6777.
Zotero 插件