Ornitz D M, Hammer R E, Davison B L, Brinster R L, Palmiter R D
Howard Hughes Medical Institute Laboratory, University of Washington, Seattle 98195.
Mol Cell Biol. 1987 Oct;7(10):3466-72. doi: 10.1128/mcb.7.10.3466-3472.1987.
An elastase-human growth hormone (hGH) fusion gene containing 205 base pairs of elastase 5' flanking region is expressed exclusively in pancreatic acinar cells of transgenic mice. This paper shows that the promoter region (-72 to +8) and the enhancer (-205 to -73) function independently of each other. The elastase enhancer can activate the heterologous mouse metallothionein gene and the hGH gene promoters; conversely, enhancers from the thymocyte-specific murine leukemia virus MCF13 and the metal regulatory elements from the metallothionein gene can activate the elastase promoter in a variety of cell types. Combinations of immunoglobulin and elastase enhancers with a heterologous promoter and the hGH gene result in expression in all of the tissues predicted by the sum of each enhancer acting alone. Thus these enhancer elements act independently of each other, suggesting that they do not have silencing activity in cells in which they are normally inactive.
一个包含205个碱基对的弹性蛋白酶5'侧翼区的弹性蛋白酶-人生长激素（hGH）融合基因仅在转基因小鼠的胰腺腺泡细胞中表达。本文表明启动子区域（-72至+8）和增强子（-205至-73）彼此独立发挥作用。弹性蛋白酶增强子可以激活异源小鼠金属硫蛋白基因和hGH基因启动子；相反，来自胸腺细胞特异性鼠白血病病毒MCF13的增强子和金属硫蛋白基因的金属调节元件可以在多种细胞类型中激活弹性蛋白酶启动子。免疫球蛋白和弹性蛋白酶增强子与异源启动子和hGH基因的组合导致在每个增强子单独作用所预测的所有组织中表达。因此，这些增强子元件彼此独立发挥作用，这表明它们在正常情况下无活性的细胞中没有沉默活性。
