Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA.
Planta. 1986 Mar;167(3):321-9. doi: 10.1007/BF00391334.
Haustoria of two palm species, Phoenix dactylifera L. (date) and Washingtonia filifera (Lindl.) Wendl were carefully dissected from seeds, and the ultrastructural characteristics of the large, electron-opaque granules present in the cells of this tissue were compared using standard aldehyde and OsO4 fixations. In Washingtonia, the granules were smaller than those in date and were more variable in size and presence of non-opaque inclusions. All granules appeared to be membrane bounded although they often filled the bounded space. No protein, lipid, carbohydrate or tannins were found in the granules by standard staining procedures. The granules stained positively with two different metallic-phosphate stains which contained either bismuth or lead. Energy dispersive X-ray microprobe analysis, done on aldehyde-fixed granules and those stained with both phosphate stains, confirmed the fact that phosphorus and calcium were present in the granules. The granules also bound the metallic stains as expected. All procedures consistently confirmed the presence of phosphate in the granules. The data are most consistent with the hypothesis that the granules are composed of polyphosphate.
两种棕榈科植物（海枣 Phoenix dactylifera L. 和华盛顿棕榈 Washingtonia filifera (Lindl.) Wendl.）的吸器从种子中小心解剖出来，并用标准的醛固定和 OsO4 固定比较了这种组织细胞中存在的大的、电子不透明颗粒的超微结构特征。在华盛顿棕榈中，颗粒比海枣小，大小和非不透明内含物的存在更具变异性。尽管颗粒经常填满边界空间，但所有颗粒似乎都有膜边界。标准染色程序未在颗粒中发现蛋白质、脂质、碳水化合物或单宁。两种不同的含有铋或铅的金属磷酸盐染色剂对颗粒进行染色呈阳性。对醛固定颗粒和用两种磷酸盐染色剂染色的颗粒进行的能量色散 X 射线微探针分析证实了颗粒中存在磷和钙的事实。颗粒也如预期的那样结合了金属染色剂。所有程序一致证实了颗粒中存在磷酸盐。这些数据最符合颗粒由多磷酸盐组成的假设。
