Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, China.
Theor Appl Genet. 1996 Jan;92(1):116-20. doi: 10.1007/BF00222960.
Genomic in situ hybridization (GISH) offers a convenient and effective method for cytological detection, but can not determine the identity of the chromosomes involved. We integrated C-banding with GISH to identify Haynaldia villosa chromosomes in a wheat background. All chromosomes of H. villosa showed C-bands, either in telomeric regions or in both telomeric and centromeric regions, which allowed unequivocal identification of each H. villosa chromosome. The seven pairs of H. villosa chromosomes were differentiated as 1-7 according to their characteristic C-bands. Using a sequential C-banding and GISH technique, we have analyzed somatic cells of F3 plants from the amphiploid Triticum aestivum-H. villosa x 'Yangmai 158' hybrids. Three plants (94009/5-4,94009/5-8 and 94009/5-9) were shown to contain H. villosa chromosome(s). 94009/5-4 (2n = 45) had three H. villosa chromosomes (2, 3 and 4); 94009/5-8 (2n = 45) possessed one chromosome 4 and a pair of chromosome 5, and 94009/5-9 (2n = 43) was found to have one chromosome 6 of H. villosa. The combination of GISH with C-banding described here provides a direct comparison of the cytological and molecular landmarks. Such a technique is particularly useful for identifying and localizing alien chromatin and DNA sequences in plants.
基因组原位杂交（GISH）为细胞学检测提供了一种便捷有效的方法，但无法确定所涉及染色体的身份。我们将 C 带与 GISH 相结合，以鉴定小麦背景中的节节麦染色体。节节麦的所有染色体均显示 C 带，要么位于端粒区，要么位于端粒和着丝粒区，这使得可以明确识别每个节节麦染色体。根据其特征 C 带，将 7 对节节麦染色体分为 1-7 号。我们使用连续 C 带和 GISH 技术，分析了双二倍体 Triticum aestivum-Haynaldia villosa x 'Yangmai 158'杂种 F3 植物的体细胞。结果表明，有 3 株（94009/5-4、94009/5-8 和 94009/5-9）含有节节麦染色体。94009/5-4（2n = 45）有 3 条节节麦染色体（2、3 和 4）；94009/5-8（2n = 45）含有 1 条 4 号染色体和 1 对 5 号染色体，而 94009/5-9（2n = 43）含有 1 条 6 号染色体。这里描述的 GISH 与 C 带结合提供了细胞学和分子标记的直接比较。该技术特别适用于鉴定和定位植物中的异源染色质和 DNA 序列。
