Bruckman Michael A, Steinmetz Nicole F
Departments of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA.
Methods Mol Biol. 2014;1108:173-85. doi: 10.1007/978-1-62703-751-8_13.
Viral nanoparticles derived from tobacco mosaic virus (TMV) find applications in various fields. We report the purification and chemical modification of TMV which is a hollow rod-shaped plant viral nanoparticle with modifiable interior and exterior surfaces. We describe methods to isolate TMV from its tobacco plant host for spatially controlled interior and exterior chemical modification and to characterize the resulting TMV hybrid materials.
源自烟草花叶病毒（TMV）的病毒纳米颗粒在各个领域都有应用。我们报告了TMV的纯化和化学修饰，TMV是一种空心杆状植物病毒纳米颗粒，其内外表面均可修饰。我们描述了从其烟草宿主中分离TMV以进行空间控制的内外化学修饰的方法，以及对所得TMV杂化材料进行表征的方法。
