Cao Binrui, Xu Hong, Mao Chuanbin
Department of Chemistry & Biochemistry, Stephenson Life Sciences Center, University of Oklahoma, Norman, OK, USA.
Methods Mol Biol. 2014;1108:123-35. doi: 10.1007/978-1-62703-751-8_10.
Phage display is a biotechnique that fuses functional peptides on the outer surface of filamentous phage by inserting DNA encoding the peptides into the genes of its coat proteins. The resultant peptide-displayed phage particles have been widely used as biotemplates for the synthesis of functional hybrid nanomaterials. Here, we describe the bioengineering of M13 filamentous phage to surface-display bone mineral (hydroxyapatite (HAP))-nucleating peptides derived from dentin matrix protein-1 and using the engineered phage as a biotemplate to grow HAP nanocrystals.
噬菌体展示是一种生物技术,通过将编码肽的DNA插入丝状噬菌体外壳蛋白的基因中,在丝状噬菌体的外表面融合功能性肽。所得的展示肽的噬菌体颗粒已被广泛用作合成功能性杂化纳米材料的生物模板。在此,我们描述了对M13丝状噬菌体进行生物工程改造,使其表面展示源自牙本质基质蛋白-1的骨矿物质(羟基磷灰石(HAP))成核肽,并使用工程化噬菌体作为生物模板来生长HAP纳米晶体。