Soto Carissa M
Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC, USA.
Methods Mol Biol. 2014;1108:155-72. doi: 10.1007/978-1-62703-751-8_12.
Viral nanoblock provides specific positioning of recognition moieties and dye molecules which can be used as a signal-generating element and enhance detection sensitivity. The methods described herein use a 30 nm viral nanoblock to couple a variety of proteins and peptides for the incorporation of recognition elements along with a large number of dye molecules (200). The bioconjugation techniques were adapted and optimized over the years to fabricate nanoparticles that exhibit high fluorescence output while maintaining the selectivity of the target receptors. These complexes can be used for the detection of pathogens and toxins in a single step since both receptor and reporter are in the same viral nanoblock. Its stability and nanometer size allows for its utilization in well-established sensing platforms like microarrays.
