Welsh Plant Breeding Station, Plas Gogerddan, Aberystwyth, Wales.
Theor Appl Genet. 1986 Apr;72(1):53-8. doi: 10.1007/BF00261454.
Galls were induced in six species of forage legumes following inoculation with wild-type strains of A. tumefaciens. The plant species was more influential than the bacterial strain in determining the type of tumour produced. Inoculation of Medicago sativa resulted in small, disorganised tumours. The three Trifolium species, T. repens, T. hybridum and T. pratense, formed galls which tended to produce roots and both Onobrychis viciifolia and Lotus corniculatus produced teratomatous galls. The shoots elongated in the latter species only. In L. corniculatus, tissues that were infected by five bacterial strains were capable of shoot regeneration when cultured on a hormone-free medium. The transformed nature of these shoots was confirmed by their failure to root, the production of callus from leaves cultured on hormone-free medium and the presence of opines.
在接种野生型根瘤农杆菌菌株后，六种饲料豆科植物上诱导出了瘿瘤。在确定产生的肿瘤类型方面，植物物种比细菌菌株的影响更大。接种紫花苜蓿导致了小而紊乱的肿瘤。三种三叶草，即白车轴草、杂三叶和红三叶，形成了倾向于产生根的瘿瘤，而苦马豆和野豌豆则产生了畸形瘿瘤。后者的芽仅在后者中伸长。在野豌豆中，当在无激素培养基上培养时，受五种细菌菌株感染的组织能够进行芽再生。这些芽的转化性质通过它们不能生根、在无激素培养基上培养的叶片产生愈伤组织以及存在冠瘿碱得到证实。
