Department of Botany and Plant Pathology, Colorado State University, 80523, Fort Collins, CO, USA.
Planta. 1983 Apr;157(5):385-91. doi: 10.1007/BF00397195.
By visual examination of calli derived from germinating seeds of wheat, oats, rice, proso millet, and pearl millet it has been possible to visually select embryogenic (E) callus which, on transfer to a regeneration medium, forms plants an average of 33 times more frequently than non-embryogenic (NE) callus of equal mass. Embryogenic callus consists of small isodiametric cells averaging 31 μm in diameter; NE callus consists of long tubular cells averaging 52 μm in width and 355 μm in length. Production of E callus is in many cases promoted by media containing 2,4-di- or 2,4,5-trichlorophenoxyacetic acid (2,4-D or 2,4,5-T) plus indole-3-acetic acid or tryptophan+kinetin. Production on NE callus is promoted by media containing 2,4-D or 2,4,5-T alone. As a result of initial experiments to optimize both media for E callus production and media for plant regeneration, callus derived in six passages from an average of 26 seeds could produce about 1,000 regenerated plants.
通过对萌发的小麦、燕麦、水稻、黍和珍珠粟种子的愈伤组织进行肉眼检查，已经可以肉眼选择出胚性（E）愈伤组织，将其转移到再生培养基上，形成植物的频率比同等质量的非胚性（NE）愈伤组织平均高 33 倍。胚性愈伤组织由平均直径为 31μm 的等径小细胞组成；NE 愈伤组织由平均宽度为 52μm、长度为 355μm 的长管状细胞组成。在许多情况下，含有 2,4-二氯苯氧基乙酸（2,4-D 或 2,4,5-T）加吲哚-3-乙酸或色氨酸+激动素的培养基可促进 E 愈伤组织的产生。仅含有 2,4-D 或 2,4,5-T 的培养基可促进 NE 愈伤组织的产生。作为优化 E 愈伤组织产生培养基和植物再生培养基的初步实验的结果，从平均 26 粒种子中经过 6 个传代的愈伤组织可产生约 1000 株再生植物。
