Institut für Züchtungsforschung Quedlinburg, Akademie der Landwirtschaftswissenschaften der DDR, DDR-4300, Quedlinburg.
Plant Cell Rep. 1984 Dec;3(6):215-8. doi: 10.1007/BF00269295.
A method for the induction of somatic embryos in soybean tissue cultures is described. Cotyledons from immature embryos were utilized as explant source. Supplementing the culture medium with auxins (2,4-D, MCPA, 2,4,5-T, NAA, IAA, IBA) caused formation of meristematic tissue on cotyledon explants. The extent of meristematic tissue formed depended on the kind and concentration of auxin in the culture medium. With 2,4-D and MCPA, embryoids originated from meristematic tissue. Embryoid formation rates were influenced by the developmental stage of the embryos serving as explant source and auxin concentration. Addition of cytokinins to the medium containing 2,4-D or supplementing it with high sugar concentrations inhibited the formation of meristematic tissue and of embryoids on cotyledon explants.
描述了一种在大豆组织培养中诱导体细胞胚的方法。以未成熟胚的子叶作为外植体来源。在培养基中添加生长素（2,4-D、MCPA、2,4,5-T、NAA、IAA、IBA）会导致子叶外植体上形成分生组织。形成的分生组织的程度取决于培养基中生长素的种类和浓度。使用 2,4-D 和 MCPA，胚状体起源于分生组织。胚状体的形成率受作为外植体来源的胚的发育阶段和生长素浓度的影响。在含有 2,4-D 的培养基中添加细胞分裂素或用高糖浓度补充它会抑制子叶外植体上分生组织和胚状体的形成。
