Programme in Genetical Research, Bose Institute, P1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India.
Plant Cell Rep. 1987 Dec;6(6):486-9. doi: 10.1007/BF00272790.
Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Regenerated plants exhibited normal appearance. These results indicate potential to introgress desirable traits from this wild crucifer into important oilseed and cole Brassicas by protoplast fusion and hybrid recovery.
从芝麻菜（Eruca sativa Lam.）的叶肉细胞中分离的原生质体在适宜的培养基上培养，进行持续的细胞分裂，形成愈伤组织。平板效率为 0.4%。原生质体衍生的愈伤组织随后通过器官发生（15.71%）和体细胞胚胎发生（11.25%）产生植株。再生植株表现出正常的外观。这些结果表明，通过原生质体融合和杂种恢复，有可能将这种野生芸薹属的优良性状导入重要的油料作物和甘蓝型油菜中。
