An G
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.
Plant Physiol. 1985 Oct;79(2):568-70. doi: 10.1104/pp.79.2.568.
Tobacco calli were transformed at levels up to 50% by cocultivation of tobacco cultured cells with Agrobacterium tumefaciens harboring the binary transfer-DNA vector, pGA472, containing a kanamycin resistance marker. Transformation frequency was dependent on the physiological state of the tobacco cells, the nature of Agrobacterium strain and, less so, on the expression of the vir genes of the tumor-inducing plasmid. Maximum transformation frequency was obtained with exponentially growing plant cells, suggesting that rapid growth of plant cells is an essental factor for efficient transformation of higher plants.
通过将烟草培养细胞与携带二元转移DNA载体pGA472的根癌农杆菌共培养，烟草愈伤组织的转化率高达50%，该载体含有卡那霉素抗性标记。转化频率取决于烟草细胞的生理状态、农杆菌菌株的性质，以及诱导肿瘤质粒的vir基因表达的影响较小。指数生长的植物细胞获得了最高的转化频率，这表明植物细胞的快速生长是高等植物高效转化的一个重要因素。
