Section of Ecology and Systematics Corson Hall, Cornell University Ithaca, 14853-2701, New York.
J Chem Ecol. 1993 May;19(5):919-37. doi: 10.1007/BF00992528.
Headspace volatiles were collected from undamaged foliage of carrot,Daucus carota, a host-plant species of the black swallowtail butterfly,Papilio polyxenes. The volatiles were fractionated over silica on an open column, and the fractions were tested in behavioral assays withP. polyxenes females in laboratory experiments. The polar fractions, as well as the total mixture of volatiles, increased the landing frequency and the number of eggs laid on model plants with leaves bearing contact-oviposition stimulants. The nonpolar fraction, containing the most abundant compounds in carrot odor, was not stimulatory. Gas Chromatographic (GC) separation of the fractions was coupled with electroantennogram (EAG) recordings to identify the compounds perceived byP. polyxenes females. The EAG activity corresponded to the behavioral activity of the fractions. None of the nonpolar compounds, identified as various monoterpenes, evoked a major EAG response, but several constituents of the polar fractions elicited high EAG responses. Sabinene hydrate (both stereoisomers), 4-terpineol, bomyl acetate, and (Z)-3-hexenyl acetate were identified by GC-MS as active compounds.
从胡萝卜（Daucus carota）未受损的叶子中收集头部空间挥发物，这是黑燕尾蝶（Papilio polyxenes）的寄主植物物种。挥发物在开放柱上用硅胶进行分离，并在实验室实验中用行为测定法对 P. polyxenes 雌性进行测试。极性馏分以及挥发性混合物的总量增加了在带有接触产卵刺激剂的叶子的模型植物上的降落频率和产卵数。非极性馏分，包含胡萝卜气味中最丰富的化合物，没有刺激性。用触角电图（EAG）记录与 GC 分离相结合，以鉴定 P. polyxenes 雌性感知的化合物。EAG 活性与馏分的行为活性相对应。未鉴定出的非极性化合物，鉴定为各种单萜烯，没有引起主要的 EAG 反应，但是极性馏分的几个成分引起了高 EAG 反应。经 GC-MS 鉴定，水合柠檬烯（两种立体异构体），4-萜烯醇，丁酸薄荷酯和（Z）-3-己烯基乙酸酯为活性化合物。
