Institute of Food and Agricultural Sciences Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, 33850, Lake Alfred, Florida.
J Chem Ecol. 1993 Jul;19(7):1293-301. doi: 10.1007/BF00984876.
The effects ofp-coumaric, ferulic, chlorogenic, and vanillic acids on photosynthesis and protein synthesis by isolated leaf cells of velvetleaf (Abutilon theophrasti Medik) were investigated. Photosynthesis and protein synthesis were measured in cell suspensions by the incorporation of(14)CO2 and [(14)C]leucine, respectively. None of the tested phenolic acids except vanillic reduced photosynthesis by more than 50% at the highest concentration and 30 min of incubation. At 100μM concentrations and 60-min incubation periods,p-coumaric, ferulic, chlorogenic, and vanillic acids inhibited photosynthesis by 33, 37, 57, and 65%, respectively. Ferulic acid was the most inhibitory to protein synthesis and reduced the incorporation of [(14)C]leucine by 50% at about 1.0μM after 60 min of incubation. At the highest concentrations tested in this study, vanillic and ferulic acids were inhibitory to photosynthesis and protein synthesis, respectively, whereas chlorogenic andp-coumaric acids did not inhibit either physiological process. The maximum inhibition of protein synthesis by chlorogenic acid was 19% and by vanillic acid was 28% at 100μM concentrations. Chlorogenic, vanillic, andp-cou-maric acids at 0.1μM caused increased protein synthesis over the untreated control. Overall, photosynthesis was more sensitive than protein synthesis to the four phenolic acids tested.
研究了对羟基肉桂酸、阿魏酸、绿原酸和香草酸对豚草（Abutilon theophrasti Medik）离体叶细胞光合作用和蛋白质合成的影响。通过（14）CO2 的掺入和（14）C 亮氨酸的掺入，分别在细胞悬浮液中测量光合作用和蛋白质合成。在所测试的酚酸中，除香草酸外，没有一种酚酸在最高浓度和 30 分钟孵育时间内使光合作用降低超过 50%。在 100μM 浓度和 60 分钟孵育期内，对羟基肉桂酸、阿魏酸、绿原酸和香草酸分别抑制光合作用 33%、37%、57%和 65%。阿魏酸对蛋白质合成的抑制作用最强，在 60 分钟孵育后，其抑制（14）C 亮氨酸掺入的浓度约为 1.0μM，达到 50%。在所测试的最高浓度下，香草酸和阿魏酸分别抑制光合作用和蛋白质合成，而绿原酸和对羟基肉桂酸则不抑制这两个生理过程。绿原酸对蛋白质合成的最大抑制率为 19%，香草酸为 28%，浓度均为 100μM。在 0.1μM 时，绿原酸、香草酸和对羟基肉桂酸引起的蛋白质合成均高于未处理的对照组。总的来说，与四种测试的酚酸相比，光合作用对四种酚酸更敏感。
