Coovadia Y M, Singh V, Bhana R H, Moodley N
J Clin Pathol. 1986 Jun;39(6):680-3. doi: 10.1136/jcp.39.6.680.
A passive haemagglutination test, using sheep red blood cells sensitised with Salmonella typhi lipopolysaccharide, was compared with the Widal test for the serological diagnosis of typhoid fever in an endemic area. The results obtained on sera from 152 patients with bacteriologically confirmed typhoid and 183 patients who did not have typhoid were analysed in terms of sensitivity, specificity, simplicity, and rapidity of the respective tests. The passive haemagglutination test was found to be more sensitive (80%) than the S typhi O antigen (71%) but marginally less sensitive than the H antigen (82%) of the Widal test. The false positive rate on control sera was 1.2% and 6.6%, respectively, for the Widal O and H antigens, and 1.6% for the passive haemagglutination test. Our findings indicate that the passive haemagglutination test is comparable with the Widal test for the serological diagnosis of typhoid fever in endemic areas, but is more simple, rapid, and economic. The passive haemagglutination test may be a useful alternative to the Widal test for the serological diagnosis of typhoid fever in busy microbiology laboratories in areas in which the disease is endemic.
在一个伤寒流行地区，将使用伤寒沙门氏菌脂多糖致敏绵羊红细胞的被动血凝试验与肥达氏试验进行比较，以用于伤寒热的血清学诊断。对152例细菌学确诊伤寒患者和183例非伤寒患者的血清检测结果，从各自检测方法的敏感性、特异性、简易性和快速性方面进行了分析。结果发现，被动血凝试验比肥达氏试验的伤寒杆菌O抗原（71%）更敏感（80%），但比肥达氏试验的H抗原（82%）稍欠敏感。肥达氏试验O和H抗原对照血清的假阳性率分别为1.2%和6.6%，被动血凝试验的假阳性率为1.6%。我们的研究结果表明，在伤寒流行地区，被动血凝试验在伤寒热的血清学诊断方面与肥达氏试验相当，但更简易、快速且经济。对于疾病流行地区繁忙的微生物实验室，被动血凝试验可能是肥达氏试验用于伤寒热血清学诊断的一种有用替代方法。
