Assadiannarenji Somayeh, Naghdi Nasser, Oryan Shahrbanoo, Azadmanesh Kayhan
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Iran J Pharm Res. 2013 Summer;12(3):457-69.
Hippocampus is essentially involved in learning and memory processes, and is known to be a target for androgen actions. The high density of the androgen receptors in hippocampus shows that there must be some relationship between androgens and memory. Androgen effects on spatial memory are complex and contradictory. Some evidence suggests a positive correlation between androgens and spatial memory. While some other reports indicated an impairment effect. The present study was conducted to assess the effect of 3α diol on spatial discrimination of rats. Adult male rats were bilaterally cannulated into CA1 region of hippocampus and then received 3α diol (0.2, 1, 3 and 6 μg/ 0.5 μL/side), indomethacin (1.5, 3 and 6 μg/ 0.5 μL/side), indomethacin (3 μg/ 0.5 μL/side) + 3α diol (1μg/ 0.5 μL/side), 25-35 min before training in Morris Water Maze task. Our results showed that injection of 3α diol (1, 3 and 6 μg/ 0.5 μL/ side) and indomethacin (3 and 6 μg/ 0.5 μL/side) significantly increased the escape latency and traveled distance to find hidden platform. It is concluded that intra CA1 administration of 3α diol and indomethacin could impair spatial learning and memory in acquisition stage. However, intra hippocampal injection of indomethacin plus 3α diol could not change spatial learning and memory impairment effect of indomethacin or 3α diol in Morris Water Maze task.
海马体主要参与学习和记忆过程，并且已知是雄激素作用的靶点。海马体中雄激素受体的高密度表明雄激素与记忆之间必定存在某种关系。雄激素对空间记忆的影响是复杂且矛盾的。一些证据表明雄激素与空间记忆呈正相关。而其他一些报告则指出存在损害作用。本研究旨在评估3α二醇对大鼠空间辨别能力的影响。成年雄性大鼠双侧海马体CA1区插管，然后在进行莫里斯水迷宫任务训练前25 - 35分钟接受3α二醇（0.2、1、3和6微克/0.5微升/侧）、吲哚美辛（1.5、3和6微克/0.5微升/侧）、吲哚美辛（3微克/0.5微升/侧）+ 3α二醇（1微克/0.5微升/侧）。我们的结果表明，注射3α二醇（1、3和6微克/0.5微升/侧）和吲哚美辛（3和6微克/0.5微升/侧）显著增加了寻找隐藏平台的逃避潜伏期和游动距离。得出的结论是，在CA1区内给予3α二醇和吲哚美辛会损害获取阶段的空间学习和记忆。然而，在海马体内注射吲哚美辛加3α二醇并不能改变吲哚美辛或3α二醇在莫里斯水迷宫任务中对空间学习和记忆的损害作用。
