Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, China.
Anal Chim Acta. 2012 Mar 9;718:84-91. doi: 10.1016/j.aca.2011.12.054. Epub 2012 Jan 2.
A novel flow injection chemiluminescence (FI-CL) sensor for determination of sulfadiazine (SDZ) using core-shell magnetic molecularly imprinted polymers (MMIPs) as recognition element is developed. Briefly, a hydrophilic MMIPs layer was produced at the surface of Fe(3)O(4)@SiO(2) magnetic nanoparticles (MNPs) via combination of molecular imprinting and reversible stimuli responsive hydrogel. And it provided the MMIPs with excellent adsorption capacity and rapid adsorption rate due to the imprinted sites mostly situated on the surface of MMIPs. Then the prepared SDZ-MMIPs were packed into flow cell to establish a novel FI-CL sensor. The sensor provided a wide linear range for SDZ of 4.0×10(-7) to 1.0×10(-4) mol L(-1) with a detection limit of 1.54×10(-7) mol L(-1). And the relative standard deviation (RSD) for the determination of 1.0×10(-6) mol L(-1) SDZ was 2.56% (n=11). The proposed method was applied to determine SDZ in urine samples and satisfactory results were obtained.
一种基于核壳型磁性分子印迹聚合物（MMIPs）作为识别元件的用于测定磺胺嘧啶（SDZ）的新型流动注射化学发光（FI-CL）传感器被开发出来。简要地说，通过分子印迹和可逆刺激响应水凝胶的结合，在 Fe(3)O(4)@SiO(2) 磁性纳米颗粒（MNPs）的表面生成了一层亲水性 MMIPs 层。由于印迹位点主要位于 MMIPs 的表面，因此该层赋予 MMIPs 以优异的吸附能力和快速的吸附速率。然后，将制备的 SDZ-MMIPs 填充到流动池中，建立了一种新型的 FI-CL 传感器。该传感器为 SDZ 提供了 4.0×10(-7) 至 1.0×10(-4) mol L(-1) 的宽线性范围，检测限为 1.54×10(-7) mol L(-1)。测定 1.0×10(-6) mol L(-1) SDZ 时的相对标准偏差（RSD）为 2.56%（n=11）。该方法被应用于测定尿样中的 SDZ，获得了令人满意的结果。
