猪突触素 1：SYN1 基因分析及启动子功能特征。
Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter.
机构信息
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, Tjele DK-8830, Denmark.
出版信息
FEBS Open Bio. 2013 Oct 7;3:411-20. doi: 10.1016/j.fob.2013.10.002. eCollection 2013.
Abstract
Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders.
摘要
突触结合蛋白 1（SYN1）是一种参与神经信号传递的磷酸化蛋白。本研究克隆并鉴定了猪 SYN1 启动子的同源物，为神经元特异性表达转基因提供了一种手段。该启动子的核苷酸序列显示了负责神经元特异性表达的元件高度保守。SYN1 的表达分析表明在胚胎发育过程中存在转录本。转绿色荧光蛋白（GFP）表达分析表明，猪 SYN1 启动子指导神经元细胞中的表达。因此，SYN1 启动子是用于生成人类神经退行性疾病猪模型的候选启动子。
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