Thiyagarajah A, Grizzle J M
J Natl Cancer Inst. 1986 Jul;77(1):141-7.
Diethylnitrosamine [(DEN) CAS: 55-18-5] caused neoplasms of the exocrine pancreas in rivulus, Rivulus ocellatus marmoratus, a self-fertilizing hermaphroditic fish, after a single exposure to 810, 270, or 130 mg DEN/liter or after a continuous exposure to 50, 17, or 9 mg DEN/liter. Rivulus developed pancreatic adenomas after a single exposure. After continuous exposure, pancreatic adenomas, cystadenomas, and adenocarcinomas developed. Adenocarcinoma developed in fish first exposed as larvae but not in those first exposed as juveniles. Adenocarcinoma of pancreatic or biliary origin invaded the intestinal muscularis layer and mucosa. Dedifferentiation of acinar cells preceded the formation of adenomas, and atypical acinar cells formed parts of some of the ductlike structures in the pancreatic neoplasms.
二乙基亚硝胺[（DEN），化学物质登记号：55-18-5] 在单剂量暴露于810、270或130毫克/升二乙基亚硝胺，或连续暴露于50、17或9毫克/升二乙基亚硝胺后，可导致自我受精的雌雄同体鱼类——眼斑溪鳉（Rivulus ocellatus marmoratus）的外分泌胰腺发生肿瘤。眼斑溪鳉在单剂量暴露后会发生胰腺腺瘤。连续暴露后，会出现胰腺腺瘤、囊腺瘤和腺癌。腺癌在幼体期首次暴露的鱼中发生，但在幼年期首次暴露的鱼中不发生。胰腺或胆管起源的腺癌侵犯肠肌层和黏膜。腺泡细胞的去分化先于腺瘤形成，非典型腺泡细胞构成了胰腺肿瘤中一些导管样结构的一部分。
