miR-15a 通过下调 Bmi-1 的表达抑制胰腺导管腺癌中的细胞增殖和上皮间质转化。
miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression.
机构信息
Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY 11794, USA.
出版信息
Cancer Lett. 2014 Mar 1;344(1):40-46. doi: 10.1016/j.canlet.2013.10.009. Epub 2013 Nov 16.
PURPOSES
To investigate whether miR-15a inhibits cell proliferation and epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PDAC) via the down-regulation of B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) expression.
METHODS AND RESULTS
miR-15a and Bmi-1 expressions in normal pancreatic tissue and PDAC tissue were measured. The relationship between miR-15a and Bmi-1 expression was analyzed. We found that miR-15a suppressed the expression of Bmi-1 and PDAC cell proliferation; E-cadherin expression was visibly up-regulated after silencing Bmi-1 by transfecting miR-15a into PDAC cell line.
CONCLUSION
miR-15a inhibits cell proliferation and EMT in PDAC via the down-regulation of Bmi-1 expression.
目的
研究 miR-15a 是否通过下调 B 细胞特异性 Moloney 鼠白血病病毒插入位点 1（Bmi-1）的表达来抑制胰腺导管腺癌（PDAC）中的细胞增殖和上皮-间充质转化（EMT）。
方法和结果
检测正常胰腺组织和 PDAC 组织中 miR-15a 和 Bmi-1 的表达情况。分析 miR-15a 与 Bmi-1 表达之间的关系。结果发现，miR-15a 抑制了 Bmi-1 的表达和 PDAC 细胞的增殖；转染 miR-15a 到 PDAC 细胞系中沉默 Bmi-1 后，E-钙黏蛋白的表达明显上调。
结论
miR-15a 通过下调 Bmi-1 的表达抑制 PDAC 中的细胞增殖和 EMT。
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