对于亚微米晶体要轻柔操作。
Gently does it for submicron crystals.
作者信息
Zeldin Oliver B, Brunger Axel T
机构信息
Oliver B Zeldin is in the Department of Molecular and Cellular Physiology and the Howard Hughes Medical Institute, Stanford University, Stanford, United States
出版信息
Elife. 2013 Nov 19;2:e01662. doi: 10.7554/eLife.01662.
Abstract
A protein structure has been refined with electron diffraction data obtained by using a very weak electron beam to collect large numbers of diffraction patterns from a few sub-micron-sized three-dimensional crystals.
摘要
利用极弱电子束从少数亚微米尺寸的三维晶体收集大量衍射图样所获得的电子衍射数据，对一种蛋白质结构进行了优化。
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3
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4
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