Coelho Willian Marinho Dourado, Gomes Jancarlo Ferreira, Amarante Alessandro Francisco Talamini do, Bresciani Katia Denise Saraiva, Lumina Giuliano, Koshino-Shimizu Sumie, Leme Denise Pereira, Falcão Alexandre Xavier
Rev Bras Parasitol Vet. 2013 Jan-Mar;22(1):1-5. doi: 10.1590/s1984-29612013000100002.
In this study, we aimed to introduce a new technique called TF-Test Modified∕Dog for the diagnosis of gastrointestinal parasites in dogs. Fecal samples from 106 dogs were processed by the technique TF-Test Modified∕Dog and the techniques of centrifugation-flotation in zinc sulfate, simple-flotation by saturated solution of sodium chloride, direct microscopy exam and TF-Test Conventional. Sensitivity was higher in the TF-Test Modified∕Dog (98.41%), followed by flotation in saturated zinc sulfate (77.78%), TF-Test Conventional (73.02%), flotation by saturated sodium chloride (55.55%), and direct microscopy exam (30.16%). The diagnostic efficiency varied from 58.49% to 99.06%, with the highest value also obtained by the new proposed technique. Efficiency level of 99.06% with kappa index 0.979 (almost perfect) was obtained with the TF-Test Modified∕Dog. These results represent significant statistical gains (P < 0.05) of 20.63% in sensitivity and 12.27% in efficiency over the best among the other techniques - flotation by saturated zinc sulfate, whose kappa index was 0.738, much lower than that of the TF-Test Modified∕Dog. All techniques presented 100% specificity. In this sense, the high sensitivity of the TF-Test Modified∕Dog makes it suitable for epidemiological surveys of gastrointestinal parasitosis in dogs, zoonoses control and preventive surveillance programs.
在本研究中，我们旨在引入一种名为改良式TF-Test∕犬的新技术，用于诊断犬类胃肠道寄生虫。对106只犬的粪便样本采用改良式TF-Test∕犬技术以及硫酸锌离心漂浮法、饱和氯化钠溶液简易漂浮法、直接显微镜检查法和传统TF-Test法进行处理。改良式TF-Test∕犬技术的敏感性最高（98.41%），其次是硫酸锌饱和溶液漂浮法（77.78%）、传统TF-Test法（73.02%）、饱和氯化钠溶液漂浮法（55.55%）和直接显微镜检查法（30.16%）。诊断效率在58.49%至99.06%之间，新提出的技术也获得了最高值。改良式TF-Test∕犬技术的效率水平为99.06%，kappa指数为0.979（几乎完美）。与其他技术中最佳的硫酸锌饱和溶液漂浮法相比，这些结果在敏感性上有显著的统计学提高（P < 0.05），提高了20.63%，在效率上提高了12.27%，硫酸锌饱和溶液漂浮法的kappa指数为0.738，远低于改良式TF-Test∕犬技术。所有技术的特异性均为100%。从这个意义上说，改良式TF-Test∕犬技术的高敏感性使其适用于犬类胃肠道寄生虫病的流行病学调查、人畜共患病控制和预防性监测计划。
