Lima Victor Fernando Santana, Cringoli Giuseppe, Rinaldi Laura, Monteiro Maria Fernanda Melo, Calado Andréa Maria Campos, Ramos Rafael Antonio Nascimento, Meira-Santos Patrícia Oliveira, Alves Leucio Câmara
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil.
Parasitol Res. 2015 Sep;114(9):3529-33. doi: 10.1007/s00436-015-4605-x. Epub 2015 Jun 28.
Dogs may be affected by different species of gastrointestinal parasites which present great importance in veterinary medicine and public health. Several techniques to diagnosing these parasites have been proposed, but different performances achieved by each method make difficult the choice of the best technique to be used. In this study, the performance of two classic methods (i.e., Willis and Hoffman techniques) and two recent techniques (i.e., FLOTAC and Mini-FLOTAC) to diagnosing gastrointestinal parasites of dogs was evaluated. Fecal samples (n = 127) of dogs divided in pools (n = 30) were collected and analyzed using four different techniques (see above). Eggs and/or oocysts of gastrointestinal parasites were detected in 93.3 % (28/30) of the samples. In particular, 20 % (6/30) were detected through the method of Hoffman, 53.3 % (16/30) by the Willis technique, and 63.3 % (19/30) and 90 % (27/30) by Mini-FLOTAC and FLOTAC, respectively. Ancylostomatidae, Trichuris vulpis and Toxocara canis were the most frequent parasites herein detected. The FLOTAC and Mini-FLOTAC techniques were the most efficient tools to detect eggs and/or oocysts of gastrointestinal parasites of dogs, therefore their use is recommended in the laboratorial routine of veterinary medicine. This study is the first report of the use of both techniques (i.e., FLOTAC and Mini-FLOTAC) to diagnosing parasites of dogs in Brazil.
犬类可能会受到不同种类的胃肠道寄生虫影响,这些寄生虫在兽医学和公共卫生中具有重要意义。已经提出了几种诊断这些寄生虫的技术,但每种方法所取得的不同性能使得选择最佳使用技术变得困难。在本研究中,评估了两种经典方法(即威利斯法和霍夫曼法)以及两种最新技术(即FLOTAC和Mini-FLOTAC)对犬类胃肠道寄生虫的诊断性能。收集了分为若干组(n = 30)的犬类粪便样本(n = 127),并使用四种不同技术进行分析(见上文)。在93.3%(28/30)的样本中检测到了胃肠道寄生虫的虫卵和/或卵囊。具体而言,通过霍夫曼法检测到20%(6/30),通过威利斯技术检测到53.3%(16/30),通过Mini-FLOTAC和FLOTAC分别检测到63.3%(19/30)和90%(27/30)。钩口科线虫、狐毛首线虫和犬弓首蛔虫是此处检测到的最常见寄生虫。FLOTAC和Mini-FLOTAC技术是检测犬类胃肠道寄生虫虫卵和/或卵囊的最有效工具,因此建议在兽医学实验室常规操作中使用。本研究是巴西首次使用这两种技术(即FLOTAC和Mini-FLOTAC)诊断犬类寄生虫的报告。