Vieira Thállitha Samih Wischral Jayme, Vieira Rafael Felipe da Costa, Nascimento Denise Amaral Gomes do, Tamekuni Kátia, Toledo Roberta Dos Santos, Chandrashekar Ramaswamy, Marcondes Mary, Biondo Alexander Welker, Vidotto Odilon
Rev Bras Parasitol Vet. 2013 Jan-Mar;22(1):104-9. doi: 10.1590/s1984-29612013000100019.
Considering the zoonotic potential of tick-borne disease (TBD) agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of TBD agents and risk factors for exposure in two different canine populations from Parana State, Southern Brazil. A total of 138 dog serum samples from urban (UA) (n=68) and rural (RA) (n=70) areas were tested with commercial ELISA rapid test for Anaplasma phagocytophilum, Ehrlichia canis and Borrelia burgdorferi antibodies and indirect immunofluorescence assay (IFAT) for Babesia vogeli. An overall of 92∕138 (66.7%) dogs, being 62∕68 (91.2%) from UA and 30∕70 (42.9%) from RA, were seropositive for at least one TBD agent. From the total number of dogs, sixty-two were positive for E. canis (44.9%), 19 (13.8%) for A. phagocytophilum, and 64 (46.4%) for B. vogeli. Anti-B. burgdorferi antibodies were not detected. Dogs from UA showed a higher percentage of tick infestation (p = 0.0135) and were highly associated with seropositivity to E. canis (p = 0.000005), A. phagocytophilum (p = 0.0001), and B. vogeli (p = 0.0012). In summary, the findings indicate that dogs from urban areas present higher potential risk exposure to TBD pathogens than those from rural areas.
考虑到蜱传疾病（TBD）病原体的人畜共患病潜力以及狗可能作为人类感染的哨兵这一事实，本研究的目的是确定巴西南部巴拉那州两个不同犬类群体中TBD病原体的血清阳性率以及暴露的风险因素。对来自城市地区（UA）（n = 68）和农村地区（RA）（n = 70）的总共138份犬血清样本进行了检测，使用商业ELISA快速检测法检测嗜吞噬细胞无形体、犬埃立克体和伯氏疏螺旋体抗体，并使用间接免疫荧光试验（IFAT）检测伯氏巴贝斯虫。总体上，92∕138（66.7%）只狗至少对一种TBD病原体呈血清阳性，其中来自UA的62∕68（91.2%）只狗和来自RA的30∕70（42.9%）只狗呈阳性。在所有狗中，62只对犬埃立克体呈阳性（44.9%），19只对嗜吞噬细胞无形体呈阳性（13.8%），64只对伯氏巴贝斯虫呈阳性（46.4%）。未检测到抗伯氏疏螺旋体抗体。来自UA的狗蜱虫感染率更高（p = 0.0135），并且与犬埃立克体（p = 0.000005）、嗜吞噬细胞无形体（p = 0.0001）和伯氏巴贝斯虫（p = 0.0012）的血清阳性高度相关。总之，研究结果表明，城市地区的狗比农村地区的狗面临更高的TBD病原体潜在暴露风险。
