Biological Experimental Station, Eötvös L. University, H-2131, Göd, Hungary.
Theor Appl Genet. 1985 Aug;70(5):548-54. doi: 10.1007/BF00305989.
Callus cultures of Nicotiana glauca, N. langsdorffii and of their tumor-forming hybrid plants contained a high frequency of cells with irregular chromosome numbers and chromosome aberrations (hypo-, hyper-, polyploid, aneuploid cells; bridges, polytene, broken, fragmented chromosomes, megachromosomes, etc.). Meristematic cells of shoot tips regenerated from the same cultures contained only regular chromosome numbers with normal chromosome structures. Variability in chromosome numbers is a consequence of abnormal mitoses. The data suggest genome segregation in the cultures. Cytological instability appears to be independent of genome segregation composition, genotype, tumorous condition, hormonal requirement and level of ploidy. The karyotype stability of the cultures is only dependent on the degree of organization of tissues and is regulated by factors involved in the control mechanisms of organizational processes.
愈伤组织培养的Nicotiana glauca、N. langsdorffii 及其肿瘤形成的杂种植物中，含有大量染色体数目不规则和染色体畸变（亚倍体、超倍体、多倍体、非整倍体细胞；桥、多线体、断裂、破碎染色体、巨大染色体等）的细胞。从同一培养物再生的茎尖分生组织细胞仅含有正常染色体数和正常染色体结构。染色体数的变异是异常有丝分裂的结果。这些数据表明在培养物中存在基因组分离。细胞学不稳定性似乎与基因组分离组成、基因型、肿瘤状态、激素需求和倍性水平无关。培养物的染色体组稳定性仅取决于组织的组织程度，并受参与组织过程控制机制的因素调节。
