Faculté des Sciences de Tunis, Laboratoire de Génétique, Tunisie.
Theor Appl Genet. 1985 Sep;70(6):577-84. doi: 10.1007/BF00252281.
Plastid and mitochondrial DNAs from Hedysarum species of the western Mediterranean basin, H. spinosissimum ssp eu-spinosissimum, H. spinosissimum ssp capitatum, H. carnosum, H. coronarium and H. flexuosum, were compared by restriction endonuclease fragment analysis. ctDNA fragment patterns for ssp eu-spinosissimum and ssp capitatum were indistinguishable in different enzyme digests. An identical ctDNA variation was found in Hpa II digests with two Sardinian populations of ssp capitatum. Each of the two subspecies was characterized by specific mt DNA patterns with Pst I, Bam HI, Sma I and EcoRI. No variation was detected in populations of different geographical origins for a given subspecies. H. carnosum, H. coronarium and H. flexuosum generated specific ct and mt DNA patterns. Comparison of mitochondrial fragments indicated: - a strong homology between the two subspecies, - a closer homology among the three other diploids, each being closer to the other two than to H. spinosissimum subspecies - as was also the case for the plastid genomes.
采用限制性内切酶片段分析法比较了来自地中海西部盆地的几种獐牙菜属（Hedysarum）植物（包括欧亚獐牙菜亚种和头状獐牙菜亚种、多枝獐牙菜、齿叶獐牙菜、鹅绒委陵菜和麻花艽）的质体和线粒体 DNA。不同酶切消化产物中亚种欧亚獐牙菜和头状獐牙菜的 ctDNA 片段模式无法区分。Hpa II 消化产物中发现的两种撒丁岛头状獐牙菜亚种的 ctDNA 变异是相同的。两个亚种均具有特有的 mtDNA 模式，分别用 Pst I、Bam HI、Sma I 和 EcoRI 进行酶切。同种亚种的不同地理起源的种群中没有检测到变异。多枝獐牙菜、鹅绒委陵菜和麻花艽产生特定的 ct 和 mtDNA 模式。线粒体片段的比较表明：- 两个亚种之间具有很强的同源性；- 其他三种二倍体之间的同源性也较高，每一种都比獐牙菜亚种更接近其他两种；- 质体基因组也是如此。
