反式二氯二氨合铂（II）使起始因子IF3与大肠杆菌30S核糖体亚基交联：16S rRNA中两个交联位点的表征；IF3可能的作用方式

Ehresmann C, Moine H, Mougel M, Dondon J, Grunberg-Manago M, Ebel J P, Ehresmann B

Nucleic Acids Res. 1986 Jun 25;14(12):4803-21. doi: 10.1093/nar/14.12.4803.

The initiation factor IF3 is platinated with trans-diamminedichloroplatinum(II) and cross-linked to Escherichia coli 30S ribosomal subunit. Two cross-linking sites are unambiguously identified on the 16S rRNA: a major one, in the region 819-859 in the central domain, and a minor one, in the region 1506-1529 in the 3'-terminal domain. Specific features of these sequences together with their particular location within the 30S subunit lead us to postulate a role for IF3, that conciliates topographical and functional observations made so far.

起始因子IF3与反式二氨二氯铂（II）发生铂化反应，并与大肠杆菌30S核糖体亚基交联。在16S rRNA上明确鉴定出两个交联位点：一个主要位点位于中央结构域的819 - 859区域，一个次要位点位于3' - 末端结构域的1506 - 1529区域。这些序列的特定特征及其在30S亚基内的特定位置使我们推测IF3具有一种作用，这种作用协调了迄今为止所做的拓扑学和功能观察。