Lührmann R, Stöffler-Meilicke M, Stöffler G
Mol Gen Genet. 1981;182(3):369-76. doi: 10.1007/BF00293924.
The location of the 3' end of 16S rRNA in E. coli 30S ribosomal subunits has been determined by immuno electron microscopy. The 3' terminal adenosine of isolated 16S rRNA was oxidized with sodium periodate and reacted with N-gamma-(2,4-dinitrophenyl) aminobutyric acid hydrazide. Functionally active 30S subunits were reconstituted from DNP-16S rRNA and total 30S ribosomal proteins. DNP-30S subunits were complexed with DNP-specific IgG-antibody and examined in the electron microscope. The 3' end of the 16S rRNA was mapped at a single region located at the inner side of the large lobe of the 30S subunit. The location of the 3' end also provides information as to the topography of the binding domain of natural mRNA on 30S subunits, since a pyrimidine-rich sequence at the 3' terminal region of 16S rRNA participates in the correct alignment of natural mRNAs during initiation complex formation.
通过免疫电子显微镜已确定了大肠杆菌30S核糖体亚基中16S rRNA 3'端的位置。分离出的16S rRNA的3'末端腺苷用过碘酸钠氧化，并与N-γ-(2,4-二硝基苯基)氨基丁酸酰肼反应。用DNP-16S rRNA和30S核糖体总蛋白重建功能活性30S亚基。DNP-30S亚基与DNP特异性IgG抗体复合，并在电子显微镜下检查。16S rRNA的3'端定位在30S亚基大叶片内侧的单个区域。3'端的位置还提供了有关天然mRNA在30S亚基上结合域拓扑结构的信息，因为16S rRNA 3'末端区域富含嘧啶的序列在起始复合物形成过程中参与天然mRNA的正确排列。
