两个相邻腺苷的二甲基化导致16S核糖体RNA二级结构不稳定。
Destabilization of secondary structure in 16S ribosomal RNA by dimethylation of two adjacent adenosines.
作者信息
Van Charldorp R, Heus H A, Van Knippenberg P H, Joordens J, De Bruin S H, Hilbers C W
出版信息
Nucleic Acids Res. 1981 Sep 11;9(17):4413-22. doi: 10.1093/nar/9.17.4413.
Abstract
Fragments comprising the 49 nucleotides from the 3'-end have been purified from 16S ribosomal RNA of wild-type Escherichia coli and from a kasugamycin-resistant mutant that specifically lacks dimethylation of two adjacent adenines near the 3'-terminus. These fragments, obtained after treatment of ribosomes in vitro with the bacteriocin cloacin DF13, were used to study the effect of the methyl groups on the temperature dependent unfolding of double-stranded regions. Both fragments contain at least 3 independent melting transitions, of which the one with the highest Tm corresponds with the unfolding of a nine-basepair long central hairpin. Dimethylation of the adenines in the loop of this hairpin lowers the melting temperature (Tm) by approximately 2 degrees C at 0.2 M NaCl and by about 5 degrees C at 0.15 M NaCl. It is suggested that m6(2)Am6(2)A is more antagonistic to loop formation that ApA and that the function of the methyl groups is to help to destabilize the 3'-terminal hairpin in 16S rRNA in order to facilitate intermolecular interactions.
摘要
已从野生型大肠杆菌的16S核糖体RNA以及一种对春日霉素耐药的突变体中纯化出包含3'-末端49个核苷酸的片段，该突变体在3'-末端附近特异性地缺乏两个相邻腺嘌呤的二甲基化。这些片段是在用细菌素氯杀菌素DF13体外处理核糖体后获得的，用于研究甲基对双链区域温度依赖性解折叠的影响。两个片段都包含至少3个独立的解链转变，其中Tm最高的一个对应于一个9个碱基对长的中央发夹的解折叠。该发夹环中腺嘌呤的二甲基化在0.2 M NaCl时使解链温度（Tm）降低约2℃，在0.15 M NaCl时降低约5℃。有人认为，m6(2)Am6(2)A比ApA对环形成的拮抗作用更强，甲基的功能是帮助使16S rRNA中的3'-末端发夹不稳定，以便促进分子间相互作用。
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