Department of Botany, The George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978, Tel Aviv, Israel.
Plant Cell Rep. 1985 Apr;4(2):70-3. doi: 10.1007/BF00269209.
Callus cultures were initiated from isolated immature embryos of Hordeum spontaneum and Hordeum bulbosum on MS or B5 basal medium supplemented with 2 mg/1 2,4-D. Shoot regeneration occurred on transfer of tissue to media containing 1 mg/1 IAA and 1 mg/1 zeatin. The regenerated shoot buds were rooted on basal medium without hormones. The in vitro regenerated plants were transferred to soil and were grown to fertile mature plants. A low percentage of albino plants was observed among the regenerated plants. No major differences were detected between the two species in respect to their potency to form callus or to the regeneration capacity. The regeneration capacity of calli decreased gradually and ended after 6 months in culture.
愈伤组织培养是从野生大麦和珠芽大麦的分离的未成熟胚上诱导的，在 MS 或 B5 基本培养基上，添加 2mg/1 的 2,4-D。将组织转移到含有 1mg/1 的 IAA 和 1mg/1 的 zeatin 的培养基上，即可进行芽再生。再生的芽在没有激素的基本培养基上生根。体外再生的植株被转移到土壤中，并生长为可育的成熟植株。在再生植株中观察到低比例的白化苗。在形成愈伤组织的能力或再生能力方面，这两个物种之间没有发现明显差异。愈伤组织的再生能力逐渐下降，培养 6 个月后结束。
