携带嵌套花椰菜花叶病毒基因组的未切割重组质粒通过基因组内重组感染植物。
Uncut recombinant plasmids bearing nested cauliflower mosaic virus genomes infect plants by intragenomic recombination.
机构信息
Biology Department C-016, University of California, San Diego, 92093, La Jolla, CA, USA.
出版信息
Plant Mol Biol. 1983 Jan;2(1):27-31. doi: 10.1007/BF00187573.
Experiments are described which show that recombinant plasmids bearing cauliflower mosaic virus genomes flanked by homologous viral DNA arms ('nested' viral genomes) can infect plants without prior excision of the CaMV genome. Infectious viral genomes are excised from such recombinant plasmids within the plant by a process of intramolecular recombination. The frequency of infection, and presumably recombination increase with the length of the homologous DNA flanking the CaMV genome. This mechanism affords us the opportunity to transfer directly the CaMV genome from bacteria to plants.
实验表明，带有花椰菜花叶病毒基因组并被同源病毒 DNA 臂包围的重组质粒（“嵌套”病毒基因组）可以在不切除 CaMV 基因组的情况下感染植物。感染性病毒基因组通过分子内重组从植物中的此类重组质粒中切除。感染频率，大概还有重组频率随 CaMV 基因组周围同源 DNA 的长度增加而增加。这种机制为我们提供了将 CaMV 基因组从细菌直接转移到植物的机会。
Zotero 插件